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NAD+ 500 mg

Original price was: 54,99 €.Current price is: 43,99 €. with VAT

NAD+ (nicotinamide adenine dinucleotide) is an essential coenzyme present in all living cells. It serves as a key electron carrier in the mitochondrial electron transport chain and as a substrate for sirtuins (SIRT1–7) and PARP enzymes responsible for DNA repair. NAD+ levels decline with age by up to 50% by middle age, making it a subject of intensive research in the fields of aging, mitochondrial biology, and neuroprotection.

Purity ≥ 98% – Each batch verified by HPLC; COA available upon request
Freeze-dried powder, store at −20 °C
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Category: Research on Longevity and the Slowing of Aging

All products are sold exclusively for the purpose of scientific research and development.
The chemical substances may not be used as a medicine, drug, active substance, medical device, cosmetic product, substance for the manufacture of a cosmetic product, or as a substance for human consumption, i.e. as a food or nutritional supplement, or in any other similar way on humans or animals.

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Description

NAD+ 500 mg — An Essential Coenzyme for Research on Cellular Energy, Aging, and DNA Repair

⚠️ Warning: This product is intended exclusively for scientific research and laboratory use. It is not intended for human or veterinary consumption, nor for the diagnosis, treatment, or prevention of any diseases. Sale restricted to individuals over the age of 18.
NAD+ (nicotinamide adenine dinucleotide) is a fundamental biological coenzyme without which cellular respiration is impossible in any living organism. It is of particular interest to researchers because its levels decline by up to 50% with age by middle age, which correlates with impaired mitochondrial function, reduced sirtuin activity, and the accumulation of DNA damage—key hallmarks of biological aging.

Chemical Identity

Parameter	Value
Full name	Nicotinamide adenine dinucleotide (oxidized form)
Abbreviation	NAD⁺
CAS Number	53-84-9
Molecular weight	663.43 Da
Molecular formula	C₂₁H₂₇N₇O₁₄P₂
Form	Freeze-dried powder
Purity	≥98% (HPLC)

Biological Functions of NAD+ Relevant to Research

1. Electron carrier in cellular respiration

NAD+ serves as an electron acceptor in glycolysis, the Krebs cycle, and beta-oxidation of fatty acids. It is reduced to NADH, which subsequently donates electrons to the electron transport chain (Complex I → III → IV) for ATP production. Without sufficient NAD+, mitochondrial respiration is limited.

2. Substrates for sirtuins (SIRT1–7) — research on aging

Sirtuins are NAD+-dependent deacetylases that regulate gene expression, stress responses, and metabolic pathways. Without NAD+, sirtuins do not function:

SIRT1 — regulation of inflammatory pathways, mitochondrial biogenesis
SIRT3 — mitochondrial homeostasis, reduction of oxidative stress
SIRT6 — DNA repair, telomere regulation

3. Substrate for PARP enzymes — DNA repair

Poly(ADP-ribose) polymerases (PARP1, PARP2) consume NAD+ during the detection and repair of single-strand DNA breaks. In models of DNA damage (UV radiation, oxidative stress), high NAD+ consumption for PARP activity is a key research phenomenon.

4. Substrates for CD38 and SARM1 — Inflammation and Neuroscience

CD38 (cyclic ADP-ribose hydrolase) and SARM1 are enzymes that degrade NAD+ in inflammatory and neurodegenerative processes. Research into their inhibition or modulation requires accessible NAD+ as a research tool.

Research Areas

Field	Related Mechanisms	State of Research
Cell Aging	Sirtuins, mitochondrial biogenesis, telomeres	Preclinical + early human studies
Mitochondrial Biology	ETC efficiency, ATP production, NAD/NADH ratio	Preclinical (in vitro + in vivo)
DNA Repair	PARP enzymes, SSB/DSB repair	Preclinical and in vitro models
Neuroprotection	SARM1 inhibition, axonal degeneration	Preclinical
Metabolic Health	Insulin sensitivity, lipolysis, adipogenesis	Preclinical + early human studies
Inflammation	CD38, NF-κB pathway	Preclinical

NAD+ vs. NMN and NR Precursors — A Research Perspective

Compound	What is	The Mechanism of NAD+ Regeneration
NAD+	Direct coenzyme	Direct delivery — highest in vitro biological activity; limited cellular permeability
NMN	Mononucleotide	Salvage pathway (NMNAT enzymes)
NR	Nucleoside	Salvage pathway (NRK enzymes)

For in vitro research (cell cultures), direct NAD+ is preferred—it allows for precise control of the concentration in the medium without relying on enzymatic conversion. For in vivo models, precursors (NMN, NR) are typically used to ensure better bioavailability.

Technical Specifications

Parameter	Value
Contents per package	500 mg
Form	Freeze-dried powder (vial)
Purity	≥98% (HPLC-verified)
Recommended solvent	Deionized water or PBS (pH 7.4)
Storage – freeze-dried	−20 °C, protect from light and moisture
Storage – after reconstitution	2–8 °C, use within 7 days; for longer storage, −80 °C
Stability	NAD+ is sensitive to hydrolysis at higher pH levels; maintain a pH of ≤ 7.4
Intended Use	Exclusively preclinical/laboratory research
Age Restriction	18+

Frequently Asked Questions About NAD+ (FAQ)

What is NAD+, and why is it the focus of aging research?

NAD+ is a coenzyme essential for mitochondrial respiration and a substrate for sirtuins—enzymes that regulate biological aging. NAD+ levels decline by up to 50% with age, which correlates with impaired cellular repair mechanisms. Research on NAD+ supplementation is one of the most active areas of longevity bioscience.

What is the difference between NAD+ and NADH?

NAD+ is the oxidized form (electron acceptor), while NADH is the reduced form (electron donor). NAD+ is generally required for research protocols—it serves as a substrate for sirtuins and PARP enzymes.

Why not use a precursor (NMN/NR) instead of NAD+ directly?

For in vitro experiments (cell cultures, enzyme assays), direct NAD+ is a more precise tool—it eliminates the variability associated with enzymatic conversion and allows for precise control of concentration.

How should NAD+ be stored?

Store the lyophilized powder at −20 °C, protected from light and moisture. After reconstitution, store at 2–8 °C (use within 7 days) or at −80 °C for long-term storage.

Is NAD+ intended for human consumption?

No. The NAD+ available on jacked.sk is intended exclusively for scientific research and laboratory use.

⚠️ Final Disclaimer

NAD+ available at jacked.sk is a research compound intended for laboratory and preclinical studies. It is not a drug, a dietary supplement, or a cosmetic product. It has not been approved by any regulatory authority for human use. The seller is not liable for any use that violates these terms and conditions.